OPTIMIZATION OF SWEETENER ISOLATION FROM STEVIA LEAVES USING RESPONSE SURFACE METHODOLOGY (RSM)

K Kamila, F Fachraniah, H Harunsyah

Sari


The optimization of sweetener isolation from stevia leaves was carried out using the Response Surface Methodology (RSM). This study aimed to optimize the isolation process of sweeteners from Stevia rebaudiana Bertoni through RSM. The independent variables used were the number of extraction cycles (A) and particle size of the material (B), with four response parameters: extract yield percentage, sweetness level (%Brix), calorie content, and sweetness intensity. The experimental design applied the Central Composite Design (CCD) with quadratic regression model analysis. The results showed that both independent variables had significant effects on the response parameters, either individually or through interaction. Finer particle size (60–70 mesh) combined with the optimal number of extraction cycles enhanced sweetness and reduced the calorie content of the extract. The optimum conditions were obtained at 70 mesh particle size and two extraction cycles, with predicted values of 6.93% yield, 5.80% Brix sweetness, 27.02 kcal/100 g calories, and a sweetness intensity of 7.08. Model validation confirmed a good agreement between predicted and actual values, indicating that RSM is effective for optimizing the sweetener isolation process from stevia leaves.

 

Keywords: Extraction, Extraction cycle, Optimization, Particle size, RSM (Response Surface Methodology), Stevia.


Teks Lengkap:

PDF (English)

Referensi


A et al., 2023)A, S. D., Ks, N., & A, L. R. (2023). Response Surface Methodology-A Statistical Tool for the Optimization of Responses. 7(1), 1–7. https://doi.org/10.19080/GJARM.2023.07.555705

Abou-Arab, E. A., Abou-Arab, A., & Abu-Salem, F. (2009). Physico-Chemical Assessment Of Natural Sweeteners Steviosides Produced From Stevia Rebudiana bertoni PLANT. Journal of Food and Dairy Sciences, 34(12), 11037–11057. https://doi.org/10.21608/jfds.2009.115819

abu bakar, E. al. (2020). Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. 1–10. https://doi.org/10.4103/jpbs.JPBS

Achyar, A. I. C. S., Ii, A., Marta, H., Dipa, D., Padjadjaran, U., & Anggaran, T. (2008). Final Research Report of the Young Researcher Program (Litmud), Universitas Padjadjaran, November, 2008.

Ahmad, J., Khan, I., Blundell, R., Azzopardi, J., & Mahomoodally, M. F. (2020). Stevia rebaudiana Bertoni.: an updated review of its health benefits, industrial applications and safety. Trends in Food Science and Technology, 100(April 2019), 177–189. https://doi.org/10.1016/j.tifs.2020.04.030

Al., M. et. (2018). Adsorption of Lead (Pb) Heavy Metal Using Lignin Isolated from Rice Straw. 03(01), 11–20.

Asworo, R. Y., & Widwiastuti, H. (2023). Influence of Simplisia Particle Size and Maceration Duration on the Antioxidant Activity of Annona muricata (Soursop) Peel Extract 3(2), 256–263. https://doi.org/10.37311/ijpe.v3i2.19906

Buchori, L. (2007). Development of a Non-Carcinogenic, Zero-Calorie Sweetener from Stevia Leaves. Reaktor, 11(2), 57. https://doi.org/10.14710/reaktor.11.2.57-60

Chatsudthipong, V., & Muanprasat, C. (2009). Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacology and Therapeutics, 121(1), 41–54. https://doi.org/10.1016/j.pharmthera.2008.09.007

Collins, S. P., Storrow, A., Liu, D., Jenkins, C. A., Miller, K. F., Kampe, C., & Butler, J. (2021). No Title 済無No Title No Title No Title. 6–8.

Diaz galuh., et al. (2022). Banana As Adsorben Adsorption Of Metal Cadmium ( Cd ) To Cadmium Sulphate ( Cdso 4 ) Using Banana Trees As. 06(200), 131–136.

Firyanto, R., Kusumo, P., & Yuliasari, I. E. (2020). Extraction of Essential Oil from Lemongrass (Cymbopogon spp.) Using the Soxhlet Extraction Method. CHEMTAG Journal of Chemical Engineering, 1(1), 1. https://doi.org/10.56444/cjce.v1i1.1252

Glikosida, P., Glikosida, S., & Glikosida, B. (n.d.). Glikon 1. O Aglikon. 1–25.

Irawan, B. (2010). Improvement of Patchouli Oil Quality through Extraction and Distillation with Various Solvent Compositions. Universitas Diponegoro. Semarang., 13.

Laili, I. N., & Amid, A. (2019). a Review on the Identification and Analysis of Heavy Metals in Herbs Using Inductively Coupled Plasma Mass Spectrometry (Icpms). Environmental Contaminants Reviews, 2(2), 01–05. https://doi.org/10.26480/ecr.02.2019.01.05

Limanto, A. (2017). Stevia: A Sugar Substitute Sweetener from the Plant Stevia rebaudiana. Kedokteran Meditek, 23(61), 1–12. https://core.ac.uk/download/pdf/326447066.pdf

Mathur, S., Bulchandani, N., Parihar, S., & Shekhawat, G. S. (2017). Critical review on steviol glycosides: Pharmacological, toxicological and therapeutic aspects of high potency zero caloric sweetener. International Journal of Pharmacology, 13(7), 916–928. https://doi.org/10.3923/ijp.2017.916.928

Maurya, A. K., & John, V. (2020). Agriculture & Food : e- Newsletter A Herbal sugar substitute – Stevia. June.

Nn, A. (2015). A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants, 04(03), 3–8. https://doi.org/10.4172/2167-0412.1000196

Nurmiah, S., Syarief, R., Peranginangin, R., & Nurtama, B. (2013). Application of Response Surface Methodology for the Optimization of Alkali Processing Conditions Treated Cottonii ( Atc ) Process Conditions Of Alkali Treated Cottonii ( Atc ) Processing. 9–22.

Pagalla, D. B. (2024). Buku ISBN Ekstraksi Bahan Alam (Issue July).

Pipit Muliyah, Dyah Aminatun, Sukma Septian Nasution, Tommy Hastomo, Setiana Sri Wahyuni Sitepu, T. (2020). Metode ekstraksi. Journal GEEJ, 7(2).

Pratama, A. (2018). Production of Low-Calorie Sugar from Stevia rebaudiana Bertoni Leaves Using the Extraction-Maceration Method

Purnamasari, A. N. R., Mubarak, A. S., & Mulyono, M. (2021). Analysis of Cadmium (Cd) Heavy Metal Using the Atomic Abrsoption Spectrophotometry (AAS) Method in Canned Crab Products at Balai Quality Testing of Fishery Products (BPMHP) Semarang, Central Java. Journal of Marine and Coastal Science, 10(2), 93. https://doi.org/10.20473/jmcs.v10i2.27661

Ritchie, L. P., & Tsichlis, O. M. (2015). Stevia: Prospects as an Emerging Natural Sweetner. Nutrition: Science, Issues, and Applications: Volume 1-2, 1–2, 764. https://doi.org/10.1201/b11391-91

Sulaiman, A. M., Hashem, H. A., & Nassar, A. G. (2022). Utilization of Stevia Leaves Powder or Stevia Leaves Aqueous Extract as a Substitute for Sugar to Produce Low Calorie Cake. Al-Azhar Journal of Agricultural Research, 47(1), 8–18. https://doi.org/10.21608/ajar.2022.266069

Sylvia, N., Sobrina, L., & Nasrun, N. (2019). Optimization of CO₂ Absorption Process Using Activated Carbon Adsorbent Computational Fluid Dynamics (CFD) dan Response Surface Methodology (RSM). Jurnal Teknologi Kimia Unimal, 8(1), 69. https://doi.org/10.29103/jtku.v8i1.1918

Tarigan, A. (n.d.). slidesaver.app_pomomx.pdf. Wölwer-Rieck, U. (2012). The leaves of Stevia rebaudiana (Bertoni), their constituents and the analyses thereof: A review. Journal of Agricultural and Food Chemistry, 60(4), 886–895. https://doi.org/10.1021/jf2044907

Zahro, H., Zaini, R. S., Nurhadianty, V., & Sarosa, A. H. (2022). Effect of Drying of Stevia rebaudiana Leaves and Number of Soxhlet Extraction Cycles on Sugar Content 6(2), 20–27.




DOI: http://dx.doi.org/10.30811/jstr.v23i02.8921

Refbacks

  • Saat ini tidak ada refbacks.


Creative Commons License

Jurnal Sains dan Teknologi Reaksi is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License

© 2016 All rights reserved |Jurnal Sains dan Teknologi Reaksi p-ISSN: 1693-248X , e-ISSN: 2549-1202.